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エチオピアのソーダ湖に生息するリムノスピラ属菌株の分子同定と培養、模擬屋外光条件下での成長評価
http://hdl.handle.net/10911/0002000416
http://hdl.handle.net/10911/0002000416f0292ade-703f-42e3-bd52-d5ac06bdd1b3
| 名前 / ファイル | ライセンス | アクション |
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rikogakukenkyuka_Ayirkm-Adugna-Woldie(sinsa).pdf (1.4 MB)
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rikogakukenkyuka_Ayirkm-Adugna-Woldie(yosi).pdf (426.6 KB)
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rikogakukenkyuka_Ayirkm-Adugna-Woldie(zen).pdf (3.7 MB)
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| Item type | 学位論文 / Thesis or Dissertation(1) | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| 公開日 | 2024-03-25 | |||||||||
| タイトル | ||||||||||
| タイトル | Molecular identification and cultivation of indigenous Limnospira strains from Ethiopian soda lakes: Growth evaluation under simulated outdoor light conditions | |||||||||
| 言語 | en | |||||||||
| タイトル | ||||||||||
| タイトル | エチオピアのソーダ湖に生息するリムノスピラ属菌株の分子同定と培養、模擬屋外光条件下での成長評価 | |||||||||
| 言語 | ja | |||||||||
| 言語 | ||||||||||
| 言語 | eng | |||||||||
| キーワード | ||||||||||
| 言語 | en | |||||||||
| 主題Scheme | Other | |||||||||
| 主題 | Biochemical composition | |||||||||
| キーワード | ||||||||||
| 言語 | en | |||||||||
| 主題Scheme | Other | |||||||||
| 主題 | Limnospira fusiformis | |||||||||
| キーワード | ||||||||||
| 言語 | en | |||||||||
| 主題Scheme | Other | |||||||||
| 主題 | molecular identification | |||||||||
| キーワード | ||||||||||
| 言語 | en | |||||||||
| 主題Scheme | Other | |||||||||
| 主題 | nutritional supplementation | |||||||||
| キーワード | ||||||||||
| 言語 | en | |||||||||
| 主題Scheme | Other | |||||||||
| 主題 | simulated outdoor light | |||||||||
| キーワード | ||||||||||
| 言語 | en | |||||||||
| 主題Scheme | Other | |||||||||
| 主題 | Shading strategies | |||||||||
| 資源タイプ | ||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_db06 | |||||||||
| 資源タイプ | doctoral thesis | |||||||||
| アクセス権 | ||||||||||
| アクセス権 | open access | |||||||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||||||
| 著者 |
Ayirkm, Adugna Woldie
× Ayirkm, Adugna Woldie
× アイリケム, アデューニ ウェルディ
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| 抄録 | ||||||||||
| 内容記述タイプ | Abstract | |||||||||
| 内容記述 | Malnutrition, especially among children with limited dietary diversity, is a significant problem in regions like Ethiopia. Arthrospira, a protein-rich cyanobacterium, could enhance nutrition through biomass production, but its use as food in Ethiopian soda lakes is underexplored. This study involved morphological isolation, growth rate screening, 16S rRNA identification, and biochemical analysis of Arthrospira-like isolates (Chapter II, Study 1). It was followed by an evaluation of the effects of varying simulated light conditions (full sunlight, greenhouse, mid-day shade in a greenhouse, and full-time shade in a greenhouse) on the growth and biochemical composition of Limnospira fusiformis strain LC-30, selected from Study 1 (Chapter III, Study 2). A total of 100 Arthrospira-like strains were isolated, with four strains exhibiting the highest specific growth rates selected for molecular identification, all confirmed as Limnospira fusiformis. These strains underwent biomass screening and biochemical analysis, showing results consistent with previous studies. Notably, strains LC-30 and LA-08 exhibited high protein and phycocyanin contents (Study1). Whole-time shade resulted in the highest protein (64.80% ash-free dry weight) and phycocyanin content (97.20 mg g⁻¹). The two-stage cultivation method for phycocyanin demonstrated a 32.7% increase in content compared to the simulated study (study 2). Identifying fast-growing Limnospira fusiformis strains and optimizing light conditions for maximizing biomass, phycocyanin and nutritional value highlights the potential of indigenous strains as nutritional supplements. | |||||||||
| 学位名 | ||||||||||
| 学位名 | 博士（工学） | |||||||||
| item_4_degree_grantor_8 | ||||||||||
| 学位授与機関名 | 創価大学 | |||||||||
| 学位授与年月日 | ||||||||||
| 学位授与年月日 | 2025-03-18 | |||||||||
| dissertation_number | ||||||||||
| 学位授与番号 | 甲第221号 | |||||||||
| 出版タイプ | ||||||||||
| 出版タイプ | VoR | |||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||
