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幹細胞におけるデルマタン硫酸の機能
http://hdl.handle.net/10911/00040737
http://hdl.handle.net/10911/00040737bebe3ca4-ca76-431e-a364-2d9865af9163
名前 / ファイル | ライセンス | アクション |
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Item type | 学位論文 / Thesis or Dissertation(1) | |||||||||
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公開日 | 2022-03-30 | |||||||||
タイトル | ||||||||||
タイトル | 幹細胞におけるデルマタン硫酸の機能 | |||||||||
言語 | ja | |||||||||
タイトル | ||||||||||
タイトル | The functions of dermatan sulphate in stem cells. | |||||||||
言語 | en | |||||||||
言語 | ||||||||||
言語 | jpn | |||||||||
キーワード | ||||||||||
言語 | en | |||||||||
主題Scheme | Other | |||||||||
主題 | mouse embryonic stem cells | |||||||||
キーワード | ||||||||||
言語 | en | |||||||||
主題Scheme | Other | |||||||||
主題 | dermatan sulphate | |||||||||
キーワード | ||||||||||
言語 | en | |||||||||
主題Scheme | Other | |||||||||
主題 | neuronal differentiation | |||||||||
キーワード | ||||||||||
言語 | en | |||||||||
主題Scheme | Other | |||||||||
主題 | undifferentiated state | |||||||||
資源タイプ | ||||||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_db06 | |||||||||
資源タイプ | doctoral thesis | |||||||||
アクセス権 | ||||||||||
アクセス権 | open access | |||||||||
アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||||||
著者 |
小倉, 千佳
× 小倉, 千佳
× Chika, Ogura
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抄録 | ||||||||||
内容記述タイプ | Abstract | |||||||||
内容記述 | Mouse embryonic stem cells (mESCs) have self-renewal and pluripotency. Various signals and growth factors maintain undifferentiated state and regulate differentiation of mESCs. Glycosylation is a one of the post-translational modifications. Glycans have various structures and are conjugated to proteins and lipids. Many proteins on the cell surface and secreted proteins are glycosylated and contribute to important biological phenomena. The signal regulation is one of them, in which signal ligands and receptors bind to specific glycan structures. Previously, we and other groups reported that heparan sulphate, one of glycosaminoglycans, contributes to maintenance of undifferentiated state and regulation of mESCs differentiation. It has been shown that chondroitin sulfate is needed for pluripotency and differentiation of mESCs, while keratan sulfate is a known marker of human ESCs / iPSCs. However, the function of dermatan sulphate (DS) in mESCs has not been fully elucidated. Here, we performed (1) induction of neuronal differentiation in mESCs and human neural stem cells adding purified DS and (2) knockdown or overexpression of the dermatan-4-O-sulfotransferase-1 (D4ST1) in mESCs. We revealed that (1) DS promotes neuronal differentiation in both mouse and human stem cells and (2) D4ST1 contributes to the undifferentiated state of mESCs. | |||||||||
学位名 | ||||||||||
学位名 | 博士(工学) | |||||||||
学位授与機関 | ||||||||||
学位授与機関名 | 創価大学 | |||||||||
学位授与年月日 | ||||||||||
学位授与年月日 | 2022-03-18 | |||||||||
学位授与番号 | ||||||||||
学位授与番号 | 甲第186号 | |||||||||
著者版フラグ | ||||||||||
出版タイプ | VoR | |||||||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 |