WEKO3
アイテム
カルモジュリンを用いたイオノクロミックナノデバイスによるバイオナノマシンの制御
http://hdl.handle.net/10911/0002000412
http://hdl.handle.net/10911/000200041285fa350d-f83c-43e1-bd10-9cdf3a56f049
| 名前 / ファイル | ライセンス | アクション |
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rikogakukenkyuka_Sabek-Yassine(sinsa).pdf (1.1 MB)
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rikogakukenkyuka_Sabek-Yassine(yosi).pdf (563.8 KB)
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rikogakukenkyuka_Sabek-Yassine(zen).pdf (3.2 MB)
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| Item type | 学位論文 / Thesis or Dissertation(1) | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| 公開日 | 2024-03-25 | |||||||||
| タイトル | ||||||||||
| タイトル | The control of bionanomachine using calmodulin-based ionoresponsive nanodevice | |||||||||
| 言語 | en | |||||||||
| タイトル | ||||||||||
| タイトル | カルモジュリンを用いたイオノクロミックナノデバイスによるバイオナノマシンの制御 | |||||||||
| 言語 | ja | |||||||||
| 言語 | ||||||||||
| 言語 | eng | |||||||||
| キーワード | ||||||||||
| 言語 | en | |||||||||
| 主題Scheme | Other | |||||||||
| 主題 | Ionoresponsive | |||||||||
| キーワード | ||||||||||
| 言語 | en | |||||||||
| 主題Scheme | Other | |||||||||
| 主題 | Calmodulin | |||||||||
| キーワード | ||||||||||
| 言語 | en | |||||||||
| 主題Scheme | Other | |||||||||
| 主題 | Small GTPase | |||||||||
| キーワード | ||||||||||
| 言語 | en | |||||||||
| 主題Scheme | Other | |||||||||
| 主題 | Ras | |||||||||
| キーワード | ||||||||||
| 言語 | en | |||||||||
| 主題Scheme | Other | |||||||||
| 主題 | GAP | |||||||||
| キーワード | ||||||||||
| 言語 | en | |||||||||
| 主題Scheme | Other | |||||||||
| 主題 | GEF | |||||||||
| 資源タイプ | ||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_db06 | |||||||||
| 資源タイプ | doctoral thesis | |||||||||
| アクセス権 | ||||||||||
| アクセス権 | open access | |||||||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||||||
| 著者 |
Sabek, Yassine
× Sabek, Yassine
× サベック, ヤシン
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| 抄録 | ||||||||||
| 内容記述タイプ | Abstract | |||||||||
| 内容記述 | Small GTPase is one of the nucleotide-driven Bionanomachine. Interestingly mechanical function and the catalytic core of small GTPase resemble ATP-driven motor proteins. Ras is a small G-protein that stimulates downstream pathways and plays an important role in cell proliferation and differentiation, involving Several factors, like GTPase-activating proteins (GAPs) and guanine nucleotide exchange factors (GEFs), regulating the activity of RAS proteins. GAPs stimulate the hydrolysis of GTP to GDP, converting RAS back to its inactive state, while GEFs promote the exchange of GDP for GTP, activating RAS. Calmodulin (CaM) is involved in intracellular signaling and regulatory events in many calcium-dependent processes in almost every eukaryotic cell. CaM target proteins have relatively short CaM-binding peptides, such as the M13 peptide, which bind tightly to the M13 peptide in the presence of calcium, activating the enzymatic activity. CaM is thus classified as an ionoresponsive molecular device. In this study. Modified and non-modified CaM were employed as regulatory devices, to dependently control Ras/Modified Ras with calcium ions as an activating factor. Firstly, CaM with GAP binding inhibitory peptide and M13 peptide, CaM-Gb-M13, was designed and prepared. The fusion protein exhibited H-Ras cycle ionoresponsive based regulation. Secondly, the modified Ras control part was designed by incorporating M13 peptide to the N-terminal and C-terminal of Ras to control by WT Calmodulin with Ca2+ as an activator. | |||||||||
| 言語 | en | |||||||||
| 学位名 | ||||||||||
| 学位名 | 博士（理学） | |||||||||
| item_4_degree_grantor_8 | ||||||||||
| 学位授与機関名 | 創価大学 | |||||||||
| 学位授与年月日 | ||||||||||
| 学位授与年月日 | 2025-03-18 | |||||||||
| dissertation_number | ||||||||||
| 学位授与番号 | 甲第218号 | |||||||||
| 出版タイプ | ||||||||||
| 出版タイプ | VoR | |||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||
