| Item type |
学位論文 / Thesis or Dissertation(1) |
| 公開日 |
2023-10-02 |
| タイトル |
|
|
タイトル |
マウス聴覚野における音誘導フラビンタンパク質自家蛍光応答と視床・皮質系入力層神経回路のニコチン性制御 |
|
言語 |
ja |
| タイトル |
|
|
タイトル |
Nicotinic regulation of tone-evoked flavoprotein autofluorescence responses and neural circuits of thalamorecipient layers in mouse auditory cortex |
|
言語 |
en |
| 言語 |
|
|
言語 |
jpn |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Nicotinic acetylcholine receptor (nAChR) |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Auditory cortex |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Sensory filtering |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Flavin autofluorescence imaging |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_db06 |
|
資源タイプ |
doctoral thesis |
| アクセス権 |
|
|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 著者 |
中西,誠
NAKANISHI, Makoto
|
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Systemic nicotine administration regulates neuronal activities in mouse auditory cortex but how nicotine regulates the spread of the activities across auditory cortical areas is not well known. We investigated this phenomenon by using flavoprotein fluorescence imaging. Nicotine exposure expanded the activated area and enhanced sound-evoked fluorescence intensity at optimal frequency peak site in AI. Intracortical administration of DHβE, an inhibitor of nicotine acetylcholine receptors composed of α4 and β2 subunits (α4β2*-nAChR), blocked nicotine-induced enhancement in the fluorescence intensity peak site, but it did not affect to the expansion of activated area. In the primary auditory system, acute nicotine exposure is known to induce sensory filtering to synchronize thalamocortical axon excitability and increase synaptic activity in AI. However, cell type and mechanism of nicotinic regulation remains unclear. We prepared in vitro auditory thalamocortical slice and investigated reduction of thalamocortical-indued action potentials in excitatory neurons in the thalamorecepient layer in AI. Moreover, we revealed a reduction in thalamocortical input to fast-spiking (FS) inhibitory cells, but not significant reduction in direct inhibitory postsynaptic potentials (IPSCs) onto cortical excitatory cells. Additionally, thalamocortical induced excitatory and inhibitory balance shifted towards excitatory dominance. These results suggest that nicotine exposure reduced cortical circuit activity but consequently increase excitatory dominance. |
|
言語 |
en |
| 学位名 |
|
|
学位名 |
博士（工学） |
| 学位授与機関 |
|
|
|
学位授与機関名 |
創価大学 |
| 学位授与年月日 |
|
|
学位授与年月日 |
2023-09-16 |
| 学位授与番号 |
|
|
学位授与番号 |
甲第203号 |
| 著者版フラグ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
kogakukenkyuka_ Makoto-NAKANISHI(sinsa).pdf
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