{"created":"2023-06-20T14:04:58.136123+00:00","id":39394,"links":{},"metadata":{"_buckets":{"deposit":"5afce7cc-3501-49c9-8d6d-5e3009937b7a"},"_deposit":{"created_by":14,"id":"39394","owners":[14],"pid":{"revision_id":0,"type":"depid","value":"39394"},"status":"published"},"_oai":{"id":"oai:soka.repo.nii.ac.jp:00039394","sets":["3813:6958:6959"]},"author_link":["91212","91213"],"item_4_date_granted_10":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2018-03-18"}]},"item_4_degree_grantor_8":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_name":"創価大学"}]}]},"item_4_degree_name_7":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士（工学）"}]},"item_4_description_6":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Haemophilus influenzae adhesin (Hia) belongs to the trimeric autotransporter family and consists of a passenger\ndomain and a transmembrane domain (HiaT). HiaT forms a 12-stranded transmembrane b-barrel. This protein has a\nunique arginine residue at 1077. Arg1077 side chains from three subunits protrude toward the center of the barrel\nand close to each other. The arrangement seems to be unfavorable for trimer assembly because of repulsion\nbetween the positive charges. To investigate the role of this residue on trimer assembly, we prepared two mutants,\nR1077M and R1077K, in which arginine at 1077 was replaced with methionine and lysine, respectively. The proteins\nwere expressed using an expression system developed in this study and purified for assembly experiments. Assembly\nexperiments indicate that the neutralization of R1077 positive charge accelerated the rate of assembly but\ndecreased the amount of the correctly assembled trimer. The result suggests that the repulsion between unique\narginine residues is important for preventing HiaT from misassembly.","subitem_description_type":"Abstract"}]},"item_4_dissertation_number_11":{"attribute_name":"学位授与番号","attribute_value_mlt":[{"subitem_dissertationnumber":"甲第158号"}]},"item_4_version_type_14":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"青木, 英莉子","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"91212","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"AOKI, Eriko","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"91213","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-04-10"}],"displaytype":"detail","filename":"sokahogaku_AOKI-Eriko(1).pdf","filesize":[{"value":"628.1 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"sokahogaku_AOKI-Eriko(1).pdf","objectType":"fulltext","url":"https://soka.repo.nii.ac.jp/record/39394/files/sokahogaku_AOKI-Eriko(1).pdf"},"version_id":"6846d963-a453-4454-b08a-16a1132757d0"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-04-10"}],"displaytype":"detail","filename":"sokahogaku_AOKI-Eriko(2).pdf","filesize":[{"value":"230.1 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"sokahogaku_AOKI-Eriko(2).pdf","url":"https://soka.repo.nii.ac.jp/record/39394/files/sokahogaku_AOKI-Eriko(2).pdf"},"version_id":"1dcfc377-fe32-4e8e-bbcb-9a735f360353"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-04-10"}],"displaytype":"detail","filename":"sokahogaku_AOKI-Eriko(3).pdf","filesize":[{"value":"18.6 MB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"sokahogaku_AOKI-Eriko(3).pdf","url":"https://soka.repo.nii.ac.jp/record/39394/files/sokahogaku_AOKI-Eriko(3).pdf"},"version_id":"a3e1cd5f-a5c9-4a03-879e-7fd37a0bc1b8"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"trimeric autotransporter","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"bacteria secretion system","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"reassembly kinetics","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"arginine cluster","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"三量体オートトランスポーターの構造形成機構に関する研究","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"三量体オートトランスポーターの構造形成機構に関する研究","subitem_title_language":"ja"},{"subitem_title":"A study on the folding and assembly mechanism of a trimeric autotransporter","subitem_title_language":"en"}]},"item_type_id":"4","owner":"14","path":["6959"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2018-04-10"},"publish_date":"2018-04-10","publish_status":"0","recid":"39394","relation_version_is_last":true,"title":["三量体オートトランスポーターの構造形成機構に関する研究"],"weko_creator_id":"14","weko_shared_id":-1},"updated":"2024-02-01T06:46:41.029803+00:00"}